MRS agar
Often abbreviated to MRS, this type of bacterial growth medium is so-named by its inventors: de Man, Rogosa and Sharpe. Developed in 1960, this medium was designed to favour the luxuriant growth of Lactobacilli for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria (although some other Lactobacillales, like Leuconostoc and Pediococcus, may grow). This medium has a clear brown colour.
Typical composition
MRS agar typically contains(w/v):
1.0 % peptone
0.8 % meat extract
0.4 % yeast extract
2.0 % glucose
0.5 % sodium acetate trihydrate
0.1 % polysorbate 80 (also known as Tween 80)
0.2 % dipotassium hydrogen phosphate
0.2 % triammonium citrate
0.02 % magnesium sulfate heptahydrate
0.005 % manganese sulfate tetrahydrate
1.0 % agar
pH adjusted to 6.2 at 25C
The yeast and meat extracts and peptone provide sources of carbon, nitrogen and vitamins for general bacterial growth. The yeast extract also contains vitamins and amino acids specifically required by Lactobacilli. polysorbate 80 is a surfactant which assists in nutrient uptake by Lactobacilli. Magnesium sulfate and manganese sulfate provide cations used in metabolism.
References
^ EMD Chemicals, MRS Agar, 2002.
^ de Man, J.D.; Rogosa, M.; Sharpe, M.E. (1960). “A Medium for the Cultivation of Lactobacilli”. J Appl Bact 23 (130-135).
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Growth media / agar plates
Selective media
Gram positive
Actinobacteria
Mycobacterium tuberculosis (Lowenstein-Jensen medium) Mycoplasma pneumoniae (Eaton’s agar)
Firmicutes
Corynebacterium diphtheriae (Hoyle’s agar) Enterococcus (Bile esculin agar) Lactobacillus (MRS agar) Staphylococcus (Mannitol salt agar)
Gram negative
Alphaproteobacteria
Brucella abortus (Brucella agar)
Betaproteobacteria
Neisseria (Thayer-Martin agar)
Gammaproteobacteria
Bordetella (Bordet-Gengou agar) Enterobacteriaceae (VRBD agar) Haemophilus influenzae/Legionella pneumophila (Buffered charcoal yeast extract agar) Pseudomonas aeruginosa (Cetrimide agar) Salmonella (XLT agar) DCA agar Salmonella/Shigella (XLD agar)
Differential media
Lactose fermenting gram negative (MacConkey agar/Sorbitol-MacConkey agar, Eosin methylene blue) Hektoen enteric agar sulfur (Bismuth sulfite agar)
Fungal media
Dermatophyte test medium Potato dextrose agar Sabouraud agar
Nonselective media
Chocolate agar Nutrient agar Plate count agar
Other/ungrouped media
Cysteine lactose electrolyte deficient agar Cystine tryptic agar Endo agar Mller-Hinton agar/PNP agar R2a agar Simmons’ citrate agar Trypticase soy agar TSI agar
Categories: Microbiological media
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