New Protandim Study Published on the National Institute of health.

Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge
This is a copy of the article and is not mine. I am a super excited distributor for Protandim and True Science. Look at some of the most interesting parts of the study and get some Protandim today!

Oxidative Medicine and Cellular Longevity
Volume 2012 (2012), Article ID 132931, 9 pages
doi:10.1155/2012/132931Research Article Phytochemical Activation of Nrf2 Protects Human Coronary Artery Endothelial Cells against an Oxidative Challenge Elise L. Donovan,1 Joe M. McCord,2 Danielle J. Reuland,1 Benjamin F. Miller,1 and Karyn L. Hamilton11Department of Health and Exercise Science, Colorado State University, 220 Moby B Complex 1582, Fort Collins, CO 80523, USA
2 Pulmonary Sciences and Critical Care Medicine, University of Colorado, Denver Anschutz Medical Campus Research Building 29th Floor, 12700 E. 19th Avenue, Aurora, CO 80045, USA Received 22 December 2011; Accepted 16 March 2012Academic Editor: Adrian Manea Copyright ? 2012 Elise L. Donovan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Translation and Future Studies of Protandim
Our data suggest positive effects of Protandim in healthy coronary artery endothelial cells supporting future examination of how Protandim may affect cells that have been chronically exposed to oxidative and lipid challenges. Most individuals experience transient and/or chronic lipid and oxidative stress in the vasculature throughout life, and it is the accumulated effects that eventually lead to overt vascular disease. Therefore, it is of interest to determine if Protandim can slow or reverse disease related endothelial cell phenotypic changes using in vivo models of chronic oxidative stress. Supplementation with Protandim in humans is safe, with no reported adverse side effects [15, 16]. It has been shown that with oral Protandim supplementation in humans, circulating TBARs, a measure of lipid peroxidation, decrease in 5-12 days, an effect that persists with continued supplementation as measured at 30 and 120 days [15]. In addition, erythrocytes isolated from subjects who ingested Protandim for 120 days had greater SOD and catalase activity compared to controls [15]. The dose ingested by human subjects [15] induces similar increases in antioxidant enzymes compared to the 20??g/mL concentration used in our experiments. Thus the effects observed in our cell culture model directly mirror those seen in vivo indicating that exposure of cells to the components of Protandim in human subjects is likely similar to what we used in vitro. Studies in vivo will need to be performed to determine if these results are translatable to intact coronary arteries.5.

Conclusion Our current investigation shows for the first time that Protandim treatment in HCAEC induces Nrf2 nuclear localization, phase II antioxidant enzyme expression, and Nrf2-dependent protection from an oxidant stress. Oxidative stress has a well-established role in CAD initiation and progression, and our data support further research on phytochemical activation of Nrf2 and the endogenous antioxidant response as a potential therapeutic approach.

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